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N. Kaun, S. Kulka, J. Frank, U. Schade, M. Vellekoop, M. Harasek, B. Lendl:
"Towards biochemical reaction monitoring using FT-IR synchrotron radiation";
Analyst, 131 (2006), 4; 489 - 494.

A lab-on-a-chip device made of CaF2 windows and SU-8 polymer was used for fluid lamination to achieve rapid mixing of two streamlines with a cross section of 300 ´ 5 mm each. Time resolved measurements of the induced chem. reaction was achieved by applying const. feeding low flow rates and by on-chip measurement at defined distances after the mixing point. Synchrotron IR microscopic detection was employed for direct and label-free monitoring of (bio)chem. reactions. Furthermore, using synchrotron IR microscopy the measurement spot could be reduced to the diffraction limit, thus maximizing time resoln. in the exptl. set-up under study. Based on computational fluid dynamic simulations the principle of the set-up is discussed. Exptl. results on the basic hydrolysis of Me chloroacetate proved the working principle of the exptl. set-up. First results on the interaction between the antibiotic vancomycin and a tripeptide (Ac2KAA) involved in the build up of the membrane proteins of gram-pos. bacteria are presented.

http://aleph.ub.tuwien.ac.at/F?base=tuw01&func=find-c&ccl_term=AC06587457